Pharmacopeia (USP), a nonprofit organization that publishes guidelines for contaminants in consumer products and the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH).Īt that high level, the FDA’s internal report said that serious adverse effects “were likely to occur,” including the potential for life-threatening issues or “permanent impairment of a body function.” The report (PDF), called a Health Hazard Evaluation, said that Johnson & Johnson’s tests detected levels of benzene 5 to 12 times above recommended limits set by two independent organizations: the U.S. The FDA published Johnson & Johnson’s statement on the agency’s website without providing any additional information about the matter.īut an internal FDA report-completed prior to the announcement and obtained by CR through the Freedom of Information Act-reached a different conclusion. The recall, Johnson & Johnson said, was being conducted “out of an abundance of caution.” The company declined to provide CR with a copy of its safety analysis. The company said its analysis found that the levels of benzene detected “would not be expected to cause adverse health effects,” even with daily exposure. In a July statement announcing the recall, Johnson & Johnson advised consumers to avoid using the affected products. The FDA confirmed Thursday that it was investigating the issue and directed manufacturers to test their products for the contaminant. Multiple aerosol personal care products from other manufacturers have since been recalled over the presence of benzene, but a broader explanation for how benzene contamination became so widespread is, for now, a mystery. At the time of the recall, Johnson & Johnson said it was investigating the matter, and a spokesperson last week provided no new information. It’s still unclear why benzene was found in the aerosol products. That’s based on independent standards organizations that say daily exposure above that threshold is associated with increased cancer risks. ![]() The FDA advises that manufacturers should limit benzene levels in consumer products and drugs to no more than 2 parts per million. The Johnson & Johnson products were the first to be recalled after the findings were released. Short-term exposure to benzene can cause dizziness and headaches, while long-term exposure causes certain types of cancer and blood disorders.Ĭoncerns about benzene in aerosol sunscreen emerged in the spring of 2021 when an independent testing organization, Valisure, released a study that detected the contaminant in dozens of those products. ![]() The products-including several of Johnson & Johnson’s Neutrogena and Aveeno aerosol sunscreens-were recalled in July after tests by the company detected benzene in multiple samples. And while Johnson & Johnson says that the levels found would not be expected to pose a health risk to consumers, the FDA report and scientists CR spoke with say otherwise. ![]() The Food and Drug Administration misled the public earlier this year about the risks posed by aerosol sunscreen products made by Johnson & Johnson that were found to be contaminated with benzene, a known carcinogen, an agency report obtained by Consumer Reports suggests.
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![]() Keep in mind that most car thieves would rather not call attention to themselves, and will go for vehicle colors like black, white, or silver, which are both more subdued colors and are more common vehicles seen on the road. ![]() The most common car colorsblack, white, silver, gray, red, and. Because of this, many retailers have focused. If a color doesn’t resonate with enough used car shoppers it will hurt resale value, even if it’s uncommon, said Brauer. Ideally, colored car wax helps camouflage scratches, but in reality, it is often difficult to find a wax color that matches your car’s paint color exactly. Porsche therefore offered the dark and very metallic Lapis Blue. Black, grey and silver are dominating the street scene since then. They’re easier to keep clean because they don’t show dirt as easily as other car colors. ![]() Ironically, it's because red cars stand out more that thieves are less likely to mess with them. Color-tinted car wax will accomplish this on micro-scratches just the same as regular wax, but it’s designed to match the color of your car. Lapis Blue Porsche 911 (2001-2008), Boxster (2001 & 2003-2007), Cayman (2006-2007), Cayenne (2003-2006) At the beginning of the new millennium, Porsche’s customers lusted after subtle colors. The easiest car colors to keep clean are white, gray, silver and blue. Yellow, red, orange, brown, and green vehicles are probably less likely to be stolen, while blue, black, silver, white, and gray are more likely to be stolen.ĭespite numerous studies suggesting evidence to the contrary, people believe that red cars are more attractive to thieves. Based on consumer choice, these car colors jockey back and forth for position like a bunch of thoroughbreds. Various shades of Red and Blue come next. White, Black, Gray, and Silver continue to top the list of best colors for cars. Audi’s own official colors are black, white and a progressive red. White, Black and various shades of grey and silver are the most popular and prevalent colors for cars. When most people see an Audi these days, they usually see one in black, gray or silver. After all, resale value is important to thieves, so they pick the popular colors. Though black, white and red are Audi’s official colors, there’s a whole palette to explore. According to Dutch economist Ben Vollaard, an assistant professor at Tilburg University in The Netherlands, mainstream-colored vehicles are more likely to be stolen, after investigating vehicle theft data in the Netherlands from 2004 through 2008. That's right: red cars' lower resale vehicles actually tends to deter thieves from breaking into red cars or stealing them. ![]() This is an incredibly powerful passive, both in the laning phase and in mid-late game as well. ![]() This effect can only occur once every 10 seconds on the same target. Tier 3: Juggernaut, though somewhat overrated due to the lack of health Zed builds, still is a 3% improvement to survivability by all means take it as the finishing point for the 9 point investment in defense.Ĭontempt For The Weak (Passive): Zed's basic attacks against targets below 50% health deal 6 / 8 / 10% of the target's maximum health as bonus magic damage. Veteran Scars is actually a massive boost to Zed's early game survivability, as it's about a 6% increase in HP at level 1. Tier 2: Unyielding essentially does what Block does, but better as it applies to spells as well. Defensive Masteries // Tier 1: Block and Recovery greatly aids Zed's early game in lane the former reduces the potency of autoattack harass, while the latter helps Zed regain health afterwards. Maximises Zed's damage potential throughout the game. Tier 5: Devastating Strikes is a must for Zed because the armor penetration stacks up with the penetration from items to give Zed a 65% armor penetation stat (assuming full stacks of Black Cleaver) as well as a small flat armor penetration. Warlord works together with his W to give Zed a massive late game damage boost. Tier 4: We take a point in Blade Weaving due to the fact that the combination of his double Razor Shuriken (from himself and his shadow) as well as his E instantly brings Zed up to the 3% maximum when full combo'ing an enemy. We take Spell Weaving mainly to get access of Blade Weaving, but the damage increase helps after you put an autoattack or two in. Executioner seems like it was made for Zed, amplifying his damage to lower health targets. Combined with his standard rune build, brings up his early bonus AD to +11. Tier 3: Martial Mastery gives Zed a good bonus to his early damage. This also synergizes with the late game bonus from his W's bonus AD amplification. Tier 2: Brute Force gives Zed a scaling bonus to his damage. We mix our 4 points in Fury and Sorcery because both are good stats for Zed and if he takes cooldown reduction glyphs the pre-game build will bring him to 10% CDR. Offensive Masteries // Tier 1: Double-Edged Sword is a must for the boost in extra damage. While he may have had his shifts of popularity in the meta, skilled Zed players will always be successful. Thus, Zed players need to have splitpushing and objective control as a constant factor for efficient usage of their time, and their team needs to respond appropriately.Īll in all, Zed is an extremely powerful assassin and is very fun and rewarding to play and master. ![]() Because of that, he is somewhat weak in teamfights apart from picking priority targets off. Unlike his AP assassin counterparts, Katarina and Akali, he lacks the repeated burst to slay entire teams, trading that power off for even faster and stronger 100-0 potential. However, not all is sunshine and flowers for the ninja of the shadows. In fact, he has one of the best waveclears in the game, and can splitpush rapidly later on in the game. He is a very safe laner, possessing a good escape mechanism as well as low-CD harass with his Razor Shuriken, and his passive allows for easy CS'ing. While his ultimate itself is countered by many means such as Quicksilver Sash and its incarnation or invulnerabilities such as Kayle's Intervention, his playmaking ability is extremely powerful, and thus only makes him stronger as your skill level with him progresses. Zed is also an extremely rewarding champion to play. While most assassins have very little escapes after blown everything to assassinate a key target and thus often die afterwards, Zed has multiple escape mechanisms that lets him contribute to a fight without getting caught up in the following debris. His whole kit revolves around dashing in, instantly and silently killing the AD or AP Carry, then instantly blinking out before any attempts to hinder him get through. Apart from the swift dashes of a Lee Sin or the graceful mobility of Ahri, many fights may feel like a simple slugfest admittedly, a great deal of tactical skill and decision-making goes into skirmishes and teamfights, but many champions simply lack the dark beauty that makes up Zed, the Master of Shadows. League is nowadays a place where true elegance is lacking. Great Room cafés on both floors are intentionally distinct from the workspaces, providing staff with a refreshing change of environment. The reception area’s spatial planning leverages magnificent views to lower Manhattan and the Hudson River, while team rooms, individual offices, collaboration spaces, and workstations make the most of tall windows and energizing city views for the whole CRA team.īright pops of color energize the workplace with back-painted laminated glass in blue and purple. This team space is set cohesively within the new office, creating a unified whole. The Elkus Manfredi team designed an open team workspace, defined spatially by its exposed industrial ceiling, with adjacent collaboration areas, and a game room that reflects the energy and work style of the Marakon team. Merging of CRA and its acquired Marakon team within the new 35th-floor workplace was also critical. The reception area features a classic Oriental rug custom over-dyed blue to balance the space’s concrete and glass aesthetic with a touch of softness. Using a semi-opaque, sound-diminishing textured glass for the conference room walls that faces reception, the design allows light into the meeting spaces while also providing visual and acoustical privacy. The reception area is immediately adjacent to three conference rooms, so it was important that it would not acoustically overpower the conference rooms. The palette was selected by the CRA team through an extensive engagement process exploring a wide range of design options.Ĭirculation spaces feature polished concrete floors the workstation spaces are carpeted in a neutral grey for a smooth visual transition from the concrete.Įfficient use of real estate was important. “An urban industrial loft aesthetic captures the essence of New York City, featuring polished concrete, exposed ceilings, dark bronze finishes, interior glass walls, and a palette of soft neutrals, punctuated by pops of blue and purple. ![]() Phenix.geometry_minimization 355d-3dna.pdb min.params You’ll need the most recently nightly build of Phenix (dev-1395 would work) and then run: Here’s a first go at refining just the backbone atoms of you input DNA model. Here is the mail reply from Jeff, using a 3DNA-generated DNA duplex ( 355d-3dna.pdb) as an example (see full details below): ![]() Particularly, Jeff Headd revised the geometry_minimization component of PHENIX to do the trick. At the meeting, I had the opportunities to talk to several members of the PHENIX team. The problem was finally solved after I attended the 43rd Mid-Atlantic Macromolecular Crystallography Meeting held at Duke University a few months ago. I tried but failed to find a satisfying third-party (command-line driven) tool that can perform restraint optimization of the sugar-phosphate backbone geometry while keeping base atoms fixed. Nevertheless, over the years, I have been concerned with the overlong O3′-P distance issue. The imprecise structure was intended as a starting point for other objectives (e.g., all-atom molecular dynamics simulations) that are out of the design scope of 3DNA. ![]() P(i+1) bond that connects consecutive nucleotides along a chain.The most noticeable effect is the long O3′(i) The backbone geometry is approximate because 3DNA uses a fixed sugar-phosphate conformation (in A- DNA, B- DNA or RNA) that is attached to the corresponding bases in the model building process. It was noted that The RMSD of reconstructed versus observed base positions is virtually zero and that for both base and backbone coordinates is <0.85 Å, even for the 146 bp nucleosomal DNA structure. In the 2003 3DNA- NAR paper, Table 3 of the section “Structures built with sugar–phosphate backbone” lists “root mean square deviation (in Å) between rebuilt 3DNA models and experimental DNA structures” for three representative DNA structures (in A-form, B-form, and a protein- DNA complex). 3DNA can build DNA/RNA structures with a precise base but approximate sugar-phosphate backbone geometry. As such their biology can be studied in more detail by looking at the fungal secretome under various circumstances and reconstructing ant infection and manipulation under controlled conditions 17. These Ophiocordyceps species can be isolated from their ant hosts and grown in vitro. Examples of this are wood ants, Formica, infected by Pandora (Phylum: Entomophthoromycota, Order Entomophthorales) 8– 10, and carpenter ants and spiny ants (tribe Camponotini, the genera Camponotus and Polyrhachis, respectively) infected by fungi of the genus Ophiocordyceps (Phylum: Ascomycota, Order Hypocreales) 11– 16. Prime examples of fungal manipulation of insect behavior are those where ants are manipulated to leave the nest and bite onto vegetation in elevated positions. ![]() Studies into the mechanisms underlying these manipulations may reveal the fungal neuromodulators that are secreted and the pathways that give rise to certain behaviors. The fungal parasites that adaptively manipulate the behavior of their insect hosts are particularly intriguing because such manipulation implies many interesting bioactive compounds are likely produced during infection 7. This imbalance is unfortunate given the many insights that could be gained from determining the mechanisms by which fungi invade and conquer their insect hosts. Despite this, fungal-insect associations remain understudied 6 in comparison to fungal parasites of plants. Many such interactions can be found with arthropods since they are the most diverse and dominant animals in all terrestrial ecosystems 4, and fungal parasites of insects (entomopathogens) can be found in all major phyla 5. Others can overcome the immune system and take advantage of the host to further transmission 3. Certain fungi have evolved to interact in mutualistic ways to provide the animal host with beneficial compounds 1, 2. From a medical perspective, this is especially true for fungi that interact with animals. Much can thus be gained from sequencing fungal genomes and discovering the enzymes and mechanisms they employ. A consequence of this broad niche space and the ability of fungi to secrete useful enzymes and bioactive compounds is their successful use in a wide variety of industries (e.g. Mutualistic and parasitic fungi evolved to intimately interact with their plant and animal hosts. Saprophytic fungi are crucial players in the carbon cycle. Our data indicate that specialized, secreted enterotoxins may play a major role in one of these strategies.įungi have evolved to occupy a wide variety of ecologically important niches. The independent co-evolution between these manipulating parasites and their respective hosts might thus have led to rather diverse strategies to alter behavior. Moreover, secondary metabolite clusters, activated during biting behavior, appeared conserved within a species complex, but not beyond. This is further supported by a relatively fast evolution of previously reported candidate manipulation genes associated with biting behavior. However, few of those proteins were conserved among them, suggesting that several different methods of behavior modification have evolved. A considerable number of (small) secreted and pathogenicity-related proteins were only found in these ant-manipulating Ophiocordyceps species, and not in other ascomycetes. These species were collected across three continents, from five different ant species in which they induce different levels of manipulation. To gain more insight into their strategies, we compared the genomes of five ant-infecting Ophiocordyceps species from three species complexes. Especially intriguing are fungal parasites that manipulate insect behavior because, presumably, they secrete a wealth of bioactive compounds. Much can be gained from revealing the mechanisms fungal entomopathogens employ. Cutwater playfully names their tequila-based libations Ranchwater, and they come in several flavors, seven to be exact. ![]() Cutwater Ranchwater Cans (aka Tequila Cocktail Cans)Īny tequila fans out there? Well, these canned cocktails are for you. There you have it three refreshing cocktails spiked with rum ready for whenever you are. But this one focuses on the classic coconut flavors of the beloved pina colada. It's high in alcohol content and high on tropical vacation vibes.Ĭutwater's Pina Colada is another cocktail in a can that contains high alcohol content. This rum cocktail, a fruity canned version of the classic cocktail, combines pineapple, coconut, citrus, and aged rum. The gently muddled mint and lime notes combine with Cutwater Bali Hai White Rum and club soda to provide a refreshing sipper. Cutwater Rum Cocktails in a CanĬutwater currently has five rum-based cocktails in a can, but our top three picks are the Rum Mojito, Tiki Mai Tai, and Pina Colada. He says that it typically falls somewhere between six months to one year.Ĭurious about how they taste? Here's our overview! We'll be looking at three flavors from each type of product. One of the most frequently asked questions about Cutwater's canned cocktails is, "how long do these last?" According to an interview with Cutwater's Master Distiller, published by Forbes, the shelf life varies from product to product. How Long Do Cutwater Canned Cocktails Last? Today, however, we are zeroing in on their fruit-based can cocktails. They are indeed a one-stop-shop for all things drinking. Yes! As we mentioned before, the Cutwater Spirits brand has its very own line of mixers, spirits, canned cocktails, and frozen cocktail pops. Let's break down some of their refreshingly satisfying flavors, shall we? But like we said, they have well over twenty options, so we may not touch every single one. ![]() But what if that fruit cocktail can come ready to go on the fly? Well, that's essentially what Cutwatter's canned cocktails are! Mixologists have constantly shaken fruity cocktails for guests, and they've always been well-loved. Now, it has a wide-ranging canned cocktail lineup, with more than twenty on offer. Cutwater can help you create plenty of cocktail recipes, from herbal liqueurs to spicy Bloody Mary mix to ginger beer. You can currently find offerings at the distillery from four different categories: spirits, canned cocktails, frozen cocktail pops, and mixers. The brand and many of its products have long lists of awards attached to them we'll check some of them out a little later on. ![]() In addition to the massive popularity with consumers, the brand is receiving good reviews from industry critics as well. According to reports, sales have increased 640 percent since last year. The company began as a side project in a brewery, broke off on its own, and was later purchased by Anhehr Busch to take advantage of nationwide distribution and marketing. One instigator of the recent success is Cutwater Spirits, which uses its own liquor in each canned cocktail. Over the last five years, canned cocktails have grown immensely popular. Read on! What Are Cutwater Canned Cocktails? Let's break down all the essential info, from calories to flavors. ![]() When prepping for your next shindig, reach for Cutwater canned cocktails for delicious ready-to-drink cocktails that come in a variety of flavors. Haven't tried canned cocktails yet? Well, Cutwater should be the one you reach for first. Who's ready to party? Nothing says party quite like fruity cocktail cans on the go. ![]() Episode 09 Young Anime Fat 7 min 360p 3D Comic: Raymond. Episode 2 Toons 2d Cartoons 7 min 360p 3D Comic: The Trainer Young Anime Bigtits 12 min 360p 3D Comic: Malevolent Intentions. 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